A comparison of the recently available genome sequences of human and mouse reveal that about 4% of the DNA is highly conserved between them. Surprisingly, only a fourth of this highly conserved DNA between these two evolutionarily distant species actually code for proteins. Much of the remainder is thought to participate in regulating gene expression. Our effort is focused toward understanding long-range regulation of genes that are important during development of the organism, and that occasionally show up in disease. Developing new technology necessary to functionally identify and understand the role of highly conserved non-coding sequences in regulating gene expression from great distances along the genome is also a major interest in the laboratory.

Shakes, L. A., Garland, D.M., Srivastava, D.K., Harewood, K.R. and Chatterjee, P. K. (2005). Minimal Cross-recombination between wild type and loxP511 sites in vivo facilitates Truncating Both Ends of Large DNA Inserts in pBACe3.6 and Related Vectors. Nucleic Acids Research. 33: e118.

Chi, X., Chatterjee, P.K., Wilson III, W., Zhang, S-X., DeMayo, F., and Schwartz, R.J. (2005). Complex Cardiac Nkx2-5 Gene Expression Activated By Noggin-Sensitive Enhancers Followed By Chamber-Specific Modules. Proc. Natl. Acad. Sci. (USA) 102: 13490-13495.

Chatterjee P.K., Mukherjee S., Shakes L.A., Wilson III, W., Harewood K.R. & Byrd G. (2004). Selecting Transpositions of a Markerless Transposon Using Phage P1 Headful Packaging: New Transposons for Functionally Mapping Long Range Regulatory Sequences in BACs. Analytical Biochemistry 335: 305-315.

Chatterjee P.K., Shakes L.A., Srivastava D.K., Garland D.M., Harewood K.R., Moore K.J. & Coren J.S. (2004). Mutually Exclusive Recombination of Wild Type and Mutant loxP Sites in vivo Facilitates Transposon-Mediated Deletions from Both Ends of Genomic DNA in PACs. Nucleic Acids Research 32: 5668-5676.

Gilmore, R.C., Baker Jr., J., Dempsey, S., Marchan, R., Corprew Jr., R.N.L., Byrd, G., Maeda, N., Smithies, O., Bukoski, R.D., Harewood, K.R. & Chatterjee, P.K. (2001). Using PAC nested-deletions to order contigs and microsatellite markers at the high repetitive sequence containing Npr3 gene locus. Gene 275:65-72.

Chatterjee, P.K., and Briley, L.P. (2000). Analysis of a Clonal Selection Event During Transposon-Mediated Nested-Deletion Formation in Rare BAC and PAC Clones. Analytical Biochemistry 285: 121-126.

Chatterjee, P.K., Yarnall, D.P., Haneline, S.A., Godlevski, M.M., Thornber , S.J., Robinson, P.S., Davies, H.E., White, N.J., Riley, J.H. and Shepherd, N.S. (1999). Direct Sequencing of Bacterial and P1 Artificial Chromosome Nested-deletions for Identifying Position-Specific Single Nucleotide Polymorphisms. Proc. Natl. Acad. Sci. (USA) 96: 13276-13281.

Chatterjee, P.K. and Coren, J.S. (1997). Isolating Large Nested Deletions in Bacterial and P1 Artificial Chromosomes by in vivo P1 Packaging of Products of Cre-catalysed Recombination Between the Endogenous and a Transposed loxP Site. Nucleic Acids Research 25: 2205-2212.

Chatterjee, P.K. and Sternberg, N. L. (1995). A General Genetic Approach in E.coli for Determining the Mechanism(s) of Action of Tumoricidal Agents: Application to DMP 840, a tumoricidal agent. Proc. Natl. Acad. Sci. (USA) 92, 8950-8954.